The UW Medical Foundation (UWMF) Professor of Pathology & Laboratory Medicine
Rm 5533, Wisconsin Institute for Medical Research (WIMR II)
1111 Highland Ave
Madison, WI 53705
- Rm 5533, Wisconsin Institute for Medical Research (WIMR II)
Cell Biology of neuronal trafficking in physiology and neurodegenerative diseases
Cell Biology of neuronal trafficking in physiology and neurodegenerative diseases. The Roy lab is interested in the cell biology of neuronal trafficking in physiology and disease. A general approach in the lab is to develop simple cellular models of normal and abnormal biological phenotypes – using dissociated neurons, 3-D organoids, and induced pluripotent stem cells (iPSC’s) – and test predictions from these models in brains. For a list of publications, please see: http://www.roylab.org/publications.html. Current projects include novel uses of CRISPR-Cas9 technology in cellular model-systems of neurodegenerative diseases, optogenetic approaches to study slow axonal transport, and use of iPSC’s to explore human cell biology. The lab has ongoing collaborations with researchers at the Wisconsin Institute for Discovery (WID), the Waisman Center, and other investigators at UW-Madison; and is located on modern, state of the art laboratory and office space overlooking lake Mendota (within the Wisconsin Institute for Medical Research or WIMR-II tower: WIMR-II-science-without-walls).
- CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage. Sun J, Carlson-Stevermer J, Das U, Shen M, Delenclos M, Snead AM, Koo SY, Wang L, Qiao D, Loi J, Petersen AJ, Stockton M, Bhattacharyya A, Jones MV, Zhao X, McLean PJ, Sproul AA, Saha K, Roy S. Nat Commun. 2019 Jan 3;10(1):53. doi: 10.1038/s41467-018-07971-8. PMID:30604771
- Processive flow by biased polymerization mediates the slow axonal transport of actin. Chakrabarty N, Dubey P, Tang Y, Ganguly A, Ladt K, Leterrier C, Jung P, Roy S. J Cell Biol. 2019 Jan 7;218(1):112-124. doi: 10.1083/jcb.201711022. Epub 2018 Nov 6. PMID:30401699
- A G, Y T, L W, et al. “A dynamic formin-dependent deep F-actin network in axons.” Journal of Cell Biology. 2015;210(3):401-417.
- Wang L, Das U, Scott DA, Tang Y, McLean PJ, Roy S. “α-synuclein multimers cluster synaptic vesicles and attenuate recycling.” Curr. Biol.. 2014;24(19):2319-26.
- Das U, Scott DA, Ganguly A, Koo EH, Tang Y, Roy S. “Activity-induced convergence of APP and BACE-1 in acidic microdomains via an endocytosis-dependent pathway.” Neuron. 2013;79(3):447-60.